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Cold Spring Harbor Protocols features standard and cutting-edge imaging laboratory techniques

October 25, 2017

While confocal microscopy relies on optical sectioning, array tomography is a volumetric microscopy method based on physical serial sectioning. Ultrathin sections of tissue are cut using an ultramicrotome, and attached in order to a glass coverslip. These coverslips are then stained as desired and imaged. The resulting two-dimensional image tiles can then be reconstructed computationally into three-dimensional volume images for visualization and quantitative analysis. The thin sections allow for rapid staining and imaging and the array format allows much of the process to be automated. Stephen J. Smith and colleagues from Stanford University (smithlab.stanford/Smithlab/Home.html) present "Array Tomography: High-Resolution Three-Dimensional Immunofluorescence," a guide to this technique that allows for visualizing previously inaccessible features of tissue structure and molecular architecture. The article is freely available on the journal's website (cshprotocols.cshlp/cgi/content/full/2010/11/pdb.top89).

Both articles are adapted from the forthcoming Imaging: A Laboratory Manual (cshlpress/link/imagingp.htm). Available later this month, the collection is the cornerstone of a new laboratory manual series, designed as an essential guide for investigators who need these visualization techniques.

Source: Cold Spring Harbor Laboratory